ABSTRACT

PCR assay for detection of mecA gene in MRSA was compared with disc diffusion method. A total of 170 strains from cancer patients undergoing chemotherapy were examined and some concordance was found in phenotypic (disc diffusion) and genotypic (PCR) methods. Coagulase producing S. aureus were further studied by using phenotypic method (slide coagulase), which revealed 141 strains as coagulase positive, while 29 remained negative. Same samples subjected to PCR-based genotypic technique, which exhibited expression of coagulase gene in all strains. A typing method for S. aureus based on PCR amplify the variable region of the coagulase gene followed by Alu I restriction enzyme digestion and analysis of restriction fragment length polymorphism (RFLP).Among genotypically positive strains of MRSA, RFLP patterns were further investigated, which showed 5 distinct electrophoretic types. Thus, this study suggests that genotypic techniques are more accurate than phenotypic methods for identification and typing of MRSA. Keywords: MRSA, Polymerase chain reaction, Restriction fragment length polymorphism, Staphylococcus aureus
Publication date: 01/01/2020

https://ijbpas.com/pdf/2020/January/MS_IJBPAS_2019_4886.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.1.4886