ABSTRACT

Oxidative stress is usually associated with the release of reactive oxygen species (ROS) involved in various pathological conditions going on inside the body. Azithromycin is a macrolide antibiotic recommended commonly for the treatment of upper respiratory infections, also acts as an active anti-inflammatory and antioxidant agent so might be beneficial in chronic inflammatory states other than infectious disease. However, at the same time it produces oxidative damages to several organs of the body due to unwanted penetration. The aim of this study is to identify suppressive role of azithromycin on ROS generation in human blood cells via chemiluminescence assay and to compare this effect with its silver and gold conjugated nano form. We have found that azithromycin suppressed ROS generation at the concentration between 250 ?g/ml and 500 ?g/ml (IC50 =310.8?g/ml). However, the maximum effect was observed with silver nanoparticle (AgNp) conjugated form of azithromycin which showed maximum effect at comparatively lower concentration (between 25?g/ml and 100?g/ml with IC50 value of 34.8?g/ml) while gold nano particle (AuNp) conjugated form remain insignificant for antioxidant activity. Although antioxidative ability is related to azithromycin alone but when it was conjugated to AgNp could lead to higher antioxidant activity in lower concentration. Therefore, the results of our studies support the antioxidant effects of azithromycin enhanced to significant levels when conjugated with silver nanoparticle as compare to azithromycin alone. Keywords: Azithromycin, antioxidant effects, Gold and silver conjugation
Publication date: 01/01/2020

https://ijbpas.com/pdf/2020/January/MS_IJBPAS_2019_4930.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.1.4930