ABSTRACT

Actinoscirpus grossus var. kysoor (Roxb.) Noltie (Family: Cyperaceae) is a perennial herb, with long stolons/ rhizomes ending in small tubers. This plant popularly known as Kasheruk in Sanskrit indravyaguna. Traditionally it was used for various medicinal purposes like a liver tonic, antidiarrhoea, anti-emetic and hepatoprotective agent and also it has some progesterone-like activity and helps in secretion of milk and development of breast. In the present research, qualitative and quantitative screening of tuber sample of plant was performed with analysis of antioxidant potential. Cold extraction method was used to prepare methanolic and chloroform extract of tuber samples. The better results were obtained in the qualitative preliminarily screening of methanolic tuber extract compared to chloroform extract. The test results showed the presence of flavonoids, alkaloids, protein, terpenoids, tannin, carbohydrate in methanolic extract. Quantitative screening of total phenolic content and total flavonoid content reported higher amount of phenolic compounds in plant i.e., 398.33±7.264 mg GAE/g of sample and 20.96±2.863 mg QE/g of sample respectively. Anti-oxidant activity of methanolic tuber extract was assessed by DPPH (diphenyl-1-picryl-hydrazyl-hydrate) radicle scavenging assay. Ascorbic acid was used as positive control. The IC50 value of positive control ascorbic acid was found to be 133.63 ± 0.41 µg/ml and that of methanolic tuber extract was found to be 305.24 ± 0.30 µg/ml. IC50 value of test sample was very nearer to standard, indicating strong antioxidant potential of plant. The plant is proven to be a good source for the preparation ofdrugs from its extract which can be very beneficial for advancement in medicine. Advance research is encouraged to understand the mechanism of plant’s biological activities and provision of value for future studies. Keywords: Actinoscirpus grossus var. kysoor, Antioxidant, Phytochemicals, Quantitative, Tuber extract
Publication date: 01/03/2022

https://www.ijbpas.com/pdf/2022/March/MS_IJBPAS_2022_5964.pdf

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https://doi.org/10.31032/IJBPAS/2022/11.3.5964