ABSTRACT

The current article highlights the estimation of quercetin in Averrhoa carambola fruits by High- Performance Thin Layer Chromatography (HPTLC). Averrhoa carambola is commonly called a Starfruit. The fruits of the plant comprise a number of constituents, mainly rich in flavonoids. Quercetin, Gallic acid and Epicatechin are the flavonoids present in starfruit. Quercetin amounts to a wide range of activities which include anti-inflammatory, anti-diabetic and also in preventing cardiovascular diseases. HPTLC is an advanced sophisticated analytical technique of thin layer chromatography. It has the primary ability to determine the accuracy and specific identification of the compounds in the plants. We used aluminium back-coated silica gel of 60F 254 stationary phase and mobile phase of Toluene: Propanol: Glacial acetic acid: n-Hexane (7:3:1:1v/v/v/v) with dosing speed of 20?L/sec and 5x5mm band length and width at 254 nm detection wavelength. Quercetin was developed in ascending mode and quantified by using JustTLC software. Rf value of quercetin was found to be 0.76. To supplement the result the developed method was validated for system suitability, accuracy, linearity, precision, LOD, LOQ and robustness according to ICH guidelines. We finally conclude that the estimation of quercetin by the HPTLC method was found to be simple, precise and accurate and can be carried out for routine analysis. Keywords: Quercetin; Averrhoa Carambola; HPTLC
Publication date: 01/04/2026

https://www.ijbpas.com/pdf/2026/April/MS_IJBPAS_2026_10090.pdf

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https://doi.org/10.31032/IJBPAS/2026/15.4.10090