The present study reports on the effect of various plant growth regulators (PGRs) on callus
induction from different types of explants of Adansonia digitata L. a globally endangered
ethnomedicinal tree. The explants viz., hypocotyl, cotyledonary leaf, leaf, root were cultured on
MS medium fortified with different concentrations (0.5–6.0 mg/L) of PGRs (2,4-D, IBA, BAP,
KIN) alone and also in combination of NAA+BAP. The highest percentage of responding cultures
from cotyledonary leaf explants (97.6% at 4.0 mg/L 2,4 D) followed by root explants (94.7% at
4.0 mg/L 2,4 D), leaf explants (93.7% at 4.0 mg/L 2,4 D) and hypocotyl explants (94.7% at
4.0 mg/L 2,4 D) were found. More amount of callus formation was also observed from all the
explants at 4.0 mg/L 2,4 D. 2, 4 D was found to be the potent PGR for callus induction and
proliferation. Whereas we have observed the shoot buds formation directly from the hypocotyl
explants at 0.5/2.0 mg/L BAP. Faster callus proliferation and very high yield of callus mass were
produced within 4 weeks on MS medium augmented with 2,4 D followed by IBA, BAP, KIN and
NAA+BAP. Callusing ability was found to be more in cotyledonary leaf cultures followed by root,
leaf and hypocotyl explants. In the present investigation, the callus development from different
explants was subcultured thrice on the fresh medium supplemented with the same concentrations and cytokinins, but we didn’t find any morphogenic event for organogenesis. Since it is
medicinally important, the protocol developed for the induction of callus can be used for isolation
of pharmaceutically important bioactive molecules in A. digitata.
Keywords: Cotyledonary leaf, Hypocotyl, Leaf, Root explants, Plant growth regulators,
Callus, A. digitata
Publication date: 01/05/2026
https://www.ijbpas.com/pdf/2026/May/MS_IJBPAS_2026_10176.pdf
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https://doi.org/10.31032/IJBPAS/2026/15.5.10176
