ABSTRACT

The present study aimed to formulate and evaluate a phytosomal gel of Punica granatum leaves for antimicrobial activity. Ethanolic extracts of Punica granatum leaves were prepared and screened for active phytoconstituents through phytochemical tests and GC-MS analysis. Phytosomes were formulated using the salting-out method with varying concentrations of soya lecithin and evaluated for entrapment efficiency, drug content, in-vitro drug release, and particle size. Among the formulations, F4 was identified as the best, exhibiting high entrapment efficiency (96.4?±?1.5%), maximum drug content (95.05?±?1.5%), and superior in-vitro drug release (96.54?±?0.57% at 10 hours). This optimized formulation was incorporated into a Carbopol 940 gel base and further evaluated for pH, viscosity (53,468 cps), spreadability, drug content (95.41%), in-vitro drug release (96.45% at 9 hours), and antimicrobial activity against Staphylococcus aureus, showing a significant zone of inhibition at a 1.0 mg concentration. The short-term stability study confirmed the formulation's stability. In conclusion, the F4 phytosomal formulation incorporated into Carbopol 940 gel demonstrated excellent physicochemical properties and enhanced antimicrobial activity, making it a promising candidate for topical applications. Keywords: Punica granatum extract, Phytosomes, Physical parameters, Phytosomal gel, Anti-microbial activity, Drug release
Publication date: 01/06/2026

https://www.ijbpas.com/pdf/2026/June/MS_IJBPAS_2026_10303.pdf

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https://doi.org/10.31032/IJBPAS/2026/15.6.10303