ABSTRACT

High performance liquid chromatography method was used to quantify rutin, quercetin and naringenin in seeds of six medicinal Trigonella species indigenous to India. For simultaneous analysis of rutin and quercetin the mobile phase was an isocratic binary system of methanol: distilled water (65:35, v/v), a flow rate of 1 ml/min, temperature of 40°C and detection wavelength of 230 nm and 298 nm respectively. For naringenin, an isocratic binary system of acetonitrile: water (65:35; v/v), a flow rate of 1 ml/min with 254 nm UV wavelength and a temperature of 40°C was used. The proposed methods were validated for analytical parameters such as accuracy, linearity, precision, robustness, limit of detection (LOD) and limit of quantification (LOQ) based on the International Conference on Harmonization (ICH) guidelines. In multiple accessions of T. foenum-graecum and T. balansae, rutin was the most abundant individual flavonoid as compared to quercetin and naringenin. In T. glabra, T. gracilis, T. occulta and T. uncata rutin could not be detected. While T. glabra, T. gracilis and T. occulta contained negligible levels of quercetin and naringenin, T. uncata displayed a flavonoid profile with the highest amount of naringenin among the tested species. The present work opens up new opportunities to explore the synergistic role of rutin with other phytoconstituents in T. foenum-graecum and T. balansae contributing to their antidiabetic potential. Keywords: India, flavonoids, indigenous, Trigonella
Publication date: 01/07/2026

https://www.ijbpas.com/pdf/2026/July/MS_IJBPAS_2026_10196.pdf

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https://doi.org/10.31032/IJBPAS/2026/15.7.10196