ABSTRACT

A chronic inflammatory condition that affects joints, arthritis causes pain, swelling, and decreased movement. Interest in medicinal plants has increased as a result of the hunt for safer and more effective substitutes for synthetic medications. In this work, the hydroalcoholic extract of the Calotropis gigantea flower a plant used in traditional medicine for its analgesic and anti-inflammatory qualities is examined for its in vitro anti-arthritic potential. A hydroalcoholic solvent system (70:30 ethanol:water) was used to create the extract, and the protein denaturation inhibition assay, which replicate the inflammatory conditions of arthritis, were used to assess the anti-arthritic effectiveness of the Calotropis gigantea flower extract. Comparable to common anti-inflammatory medications like diclofenac sodium, the extract was shown to dramatically reduce protein denaturation in a dose-dependent way. Flavonoids, alkaloids, tannins, and saponins were found by phytochemical screening and may be responsible for the activity that was seen. According to the results, the hydroalcoholic extract of the flower of the Calotropis gigantea shows encouraging anti-arthritic properties and may be used as a natural treatment for arthritis. To confirm its effectiveness and clarify the underlying mechanisms, more in vivo research and molecular investigations are necessary. Keywords: Anti-arthritic, Calotropis gigantea, denaturation, diclofenac sodium, in vitro, protein
Publication date: 01/07/2026

https://www.ijbpas.com/pdf/2026/July/MS_IJBPAS_2026_10301.pdf

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https://doi.org/10.31032/IJBPAS/2026/15.7.10301