Benign Prostatic Hyperplasia (BPH) and Overactive Bladder (OAB) are common urological
disorders in older men. Managing these conditions often requires a combination therapy that
includes Mirabegron, a ?3-adrenergic receptor agonist, and Silodosin, a selective ?1A-
adrenergic receptor antagonist. These drugs work well together to address both storage and
voiding symptoms.
Effective, inexpensive, and eco-friendly methods are needed to measure these drugs in
pharmaceutical formulations. This ensures they work properly and meet regulations.
Traditional methods like stability-indicating RP-HPLC, are precise, sensitive, and strong.
However, they use toxic organic solvents and produce a lot of waste, creating serious
environmental issues. Therefore, greener options are necessary.
Green Analytical Chemistry focuses on developing methods that use less solvent, produce less
hazardous waste, and use resources more efficiently. In this setting, UV spectrophotometry
stands out as a promising option. It provides a simple, eco-friendly, and cost-effective way to
measure both drugs at the same time.
Importantly, evaluations using greenness metrics, such as the Analytical Eco-Scale, Green
Analytical Procedure Index (GAPI), and Analytical Greenness metric, showed that the UVmethod has a better environmental profile than HPLC. The Eco-Scale scored 96 for UV and 87
for HPLC, confirming its environmental advantage.
Additionally, statistical tests using Student’s t-test and F-test found no significant difference (p
> 0.05) between the two methods, supporting the UV approach as a valid alternative. Overall,
this review shows that UV spectrophotometry is not only scientifically reliable but also
environmentally sustainable. It serves as a dependable tool for routine quality control of
Mirabegron and Silodosin while supporting global sustainability goals in pharmaceutical
analysis.
Keywords: Mirabegron and Silodosin, ?3-adrenergic receptor, UV, HPLC, GAPI, AGREE
Publication date: 01/07/2026
https://www.ijbpas.com/pdf/2026/July/MS_IJBPAS_2026_10341.pdf
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https://doi.org/10.31032/IJBPAS/2026/15.7.10341
