ABSTRACT

Validation is a documented program that provides a high degree of assurance that a facility or operation will consistently produce product meeting a predetermined specification. The present paper illustrates about development and validation of a new, simple, precise and accurate UPLC method for the determination of the paroxetine and clonazepam in bulk and its dosage forms. Mobile phase composition is acetonitrile, methanol and buffer were mixed in the ratio of 10:50:40. Linearity of the method was established by analysis of mixed standard solution containing 250-750 ?g/ml for paroxetine and 10-30 ?g/ml for clonazepam. The calibration curves drawn by plotting the response versus concentration were found to be linear and their coefficient of correlations (R2) values are 0.9993 and 0.9997 for paroxetine and clonazepam respectively. The good percentage recovery of the sample clearly indicates. The reproducibility and accuracy of the developed method. Similarly, the RSD value for precision was found to be within acceptable limit. The method can be useful for the day-to-day routine analysis in the quality control departments of bulk and pharmaceutical formulations industries. Keywords: Paroxetine, clonazepam, benzodiazepine receptors, validation, linearity
Publication date: 01/07/2026

https://www.ijbpas.com/pdf/2026/July/MS_IJBPAS_2026_10346.pdf

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https://doi.org/10.31032/IJBPAS/2026/15.7.10346