ABSTRACT

The aim of the present research work is to develop and validate methods for the simultaneous estimation of doxycycline hyclate and colistin sulphate from a mixture, by using UV spectroscopy and High-Performance liquid chromatography. UV spectroscopic Absorption correction method using water as a solvent was developed. Chromatographic separation was performed on a Zorbax XDB C18 column packed with particles of 5? and dimensions of 150*4.6 mm. The acceptable separation was achieved using a mobile phase gradient for a run time of 18 minutes. The mobile phase A consist of water: acetonitrile: formic acid (90:10:0.08 v/v/v) while mobile phase B was water: acetonitrile: formic acid (95:05:0.1 v/v/v) and was delivered at a flow rate of 1ml/min. The detection was made at 210 nm. The linearity of the method was found to be 0.9996 and 0.9995 for doxycycline hyclate and colistin sulphate respectively for HPLC. It was 0.9998 at 338nm and 0.9992 at 202nm for UV. The limit of detection and limit of quantitation values of doxycycline hyclate and colistin sulphate found to be 30.921 ?g/ml, 93.701 ?g/ml and 17.544 ?g/ml, 53.164 ?g/ml, respectively for HPLC. Both the methods were found to be accurate, robust and precise. Keywords: Doxycycline hyclate, Colistin sulphate, UV-visible spectroscopy, HPLC, method development
Publication date: 01/08/2026

https://www.ijbpas.com/pdf/2026/August/MS_IJBPAS_2026_10410.pdf

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https://doi.org/10.31032/IJBPAS/2026/15.8.10410