ABSTRACT

Salbutamol (albuterol) is an extensively administered agonist of the beta 2-adrenergic receptor also used in the treatment of bronchospasm of asthma and chronic obstructive pulmonary disease (COPD) [1]. Due to the pharmacological significance of salbutamol and its widespread use in clinical practice, the need of precise, accurate and validated methods of analysis has turned out to be significant to quality control and regulatory assurance [4]. High-Performance Liquid Chromatography (HPLC) is one of the most frequently used and, moreover, it is highly sensitive and reproducible. HPLC allows the successful quantification of both bulk and formulated form of drug [1]. High-Performance Thin Layer Chromatography (HPTLC) provides a low-cost and fast method of routine analysis, particularly well suited to qualitative identification, and stability studies [1]. Salbutamol is not volatile and so Gas Chromatography (GC) is not used commonly, but it becomes efficient with derivatization methods increasing its detectability in complex mixtures [2]. Liquid Chromatography-Mass Spectrometry (LC-MS) takes the benefits of both excellent separation capacity of liquid chromatography and the high specificity and sensitivity of mass spectrometry; thus, it is suitable in pharmacokinetic and bioanalytical applications of biological samples such as plasma and urine [1]. Ultraviolet (UV) spectroscopy is also an important measure used in the initial examinations because of its affordability, and simplicity of use. It is widely applied in ascertaining the concentration ofdrugs in their formulas by calculating absorbance at its specific wavelength (?max ~ 276 nm) [1]. This combination of analytical methodologies guarantees thorough analysis of the purity, contents, stability and possible degradation products of salbutamol. The various methods have their own merits which are applied in analysis depending on the requirements of the study, i.e., quality control routine or advanced pharmacokinetics research implementation, and therefore play their part in the proper therapeutic use of salbutamol [3, 4]. Keywords: Spectroscopic And Chromatographic Methods, Salbutamol Analysis, Pharmaceutical Formulations, Biological Samples
Publication date: 01/09/2026

https://www.ijbpas.com/pdf/2026/September/MS_IJBPAS_2026_10356.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2026/15.9.10356