ABSTRACT

Background: The phytochemical evaluation of the methanolic extract of the plant, Neolamarckia macrophylla. (Rubiaceae- madder family) was carried out to evaluate its therapeutic potential. Despite being part of traditional medicine, not much research has been done on the plant. Materials and Methods: The study aimed to understand the plant’s phytochemistry and evaluate its therapeutic potential. For this purpose, DPPH, FRAP compared to ascorbic acid, Total Antioxidant Assay, AChE inhibition Assay, and HR-LCMS techniques were employed. Results & discussion: Major secondary metabolites in the methanolic extract were flavonoids, tannins, phenolics, and steroids. The free radical scavenging capacity of the methanolic extract of the plant was evaluated using four different methods, proving the plant to possess high antioxidant properties. Hence, in vitro anti Alzheimer’s activity of methanolic extract was evaluated using an AChE inhibition assay based on Ellins method. Conclusion: When treated with methanolic extract, the percentage ofinhibition was found to be 81.71% at 500 ?g/mL. concentration. IC50 for methanolic extract was attained at 118.6 ?g/mL. Also, chemical characterization of the extract was performed using the HR-LCMS technique. Furthermore, in a Scopolamine-Induced Amnesia rat Model, methanolic extract demonstrated anti-Alzheimer’s potential, promising natural alternative or adjunct to conventional cholinesterase inhibitors in the management of Alzheimer’s disease. Keywords: Neolamarckia macrophylla, Rubiaceae- madder, Antioxidant assay, HR-LC MS, AChE inhibition assay, Anti Alzheimer’s
Publication date: 01/09/2026

https://www.ijbpas.com/pdf/2026/September/MS_IJBPAS_2026_10701.pdf

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https://doi.org/10.31032/IJBPAS/2026/15.9.10701