The creation and assessment of a multipurpose polyherbal face serum enhanced with natural extracts
such as fenugreek, cucumber, rice, guava leaves, and aloe vera are the main objectives of the present
study. The formulation sought to preserve stability and safety for cosmetic use while offering
antibacterial, antioxidant, and skin-nourishing properties. Numerous criteria, including pH, viscosity,
spreadability, stability, and biological activity, were evaluated for the produced formulations. Among
the developed formulations, F3 exhibited superior performance. in the test, exhibiting excellent
antibacterial activity against both Gram-positive (Staphylococcus aureus) and Gram-negative (E. coli)
bacteria, appropriate pH (4.1–6.7), and improved spreadability (7.3 g.cm/s). Additionally, with a zone
of inhibition similar to that of common antifungal drugs, the serum demonstrated exceptional antifungal
activities, especially against Aspergillus niger. F3 had the highest hydrogen peroxide scavenging ability
(85%), according to an antioxidant activity study, which suggests that it could be a good option for
lowering oxidative stress on the skin. The serum's endurance was guaranteed by stability tests conducted
over a month, which showed no phase separation or physical alterations. Its safety for topical use was
confirmed by skin irritation tests that revealed no erythema, oedema, or negative reactions. Excellent
stability, antimicrobial, and antioxidant qualities were demonstrated by the developed herbal face serum
formulation, especially F3, while preserving a pH that was suitable for the skin. These results imply
that the created serum is both safe and effective for use in skincare products.
Keywords: Herbal face serum, Antioxidant activity, antimicrobial properties, fenugreek seeds,
guava leaves, rice water
Publication date: 01/06/2026
https://www.ijbpas.com/pdf/2026/June/MS_IJBPAS_2026_10247.pdf
Download PDF
https://doi.org/10.31032/IJBPAS/2026/15.6.10247
